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Objective:Searching after the effects of the expressions the neonatal rabbits with chronic intrauterine anoxemia give to neutrophil and conglutinated molecule.Method:Choosing 15 Zelanian rabbits that have been in afternoon pregnancy,we divide them into three stochastic contratest groups by chance:the control group,the anoxic group and the group interfered with Chinese traditional medicine.Then,we contrast the expressions and changes of these groups with CD11b/CD18,ICAM-1 and VCAM-1.Results:Contrasting the CD11b/CD18,ICAM-1 and VCAM-1 of the anoxic group and the group interfered with Chinese traditional medicine with chinese traditional medicine with the control group,we have found their expressions all more remarkably strengthened;There is some difference in expression between the anoxic group and the group interfered with Chinese traditional medicine,but it has little statistical significance.Conclusion:In this article,there occurs the mention:the chemotaxis of neutrophil has been strengthened and the endotheliocytosis of cerebrovascular has been conglutinated under anoxemia;as a result,the chemotaxis and endotheliocytosis have marred the brain cell tissue and participated in whole pathological and physiological process.
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@#Objective To investigate the changes of adhesion molecules and their effects on skeletal muscle ischemia/reperfusion injury. Methods 42 Wistar rats were divided into 3 groups: normal control group (Group Ⅰ, n=6), ischemia group (Group Ⅱ, n=6),ischemia/reperfusion injury group (Group Ⅲ, n=30). The level of malondialdehyde (MDA) in the plasma, myeloperoxidase (MPO) in the skeletal muscle, CD11b/CD18 on the leukocytes, intercelluar adhesion molecule-1 (ICAM-1) in the skeletal muscle and the histological changes were studied 1 h, 2 h, 4 h, 8 h, 12 h reperfusion after ischemia for 4 h. Results In group Ⅲ, the expression of CD11b/CD18, ICAM-1 and the injury of skeletal muscle increased with the lapse of reperfusion time. They reached the peak at 8～12 hours' reperfusion. The injury of skeletal muscle developed with the expression of adhesion molecules. Conclusion The expression of CD11b/CD18 and ICAM-1 are significantly associated with the skeletal muscle ischemia-reperfusion injury.
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Objective:Explore the relationship between AMS expression and skin damage.Metheds:The study employed ELISA method,immunohistochemical method and electron minroscope analysis.Results:The results showed that the index of EC-ICAM-1 and PMN-CD11b plsitively expression increased remarkably in class 2~3 compared with that in class 1 and contrast group.The index of PMN-CD18 expression in class 2~3 and class 1 was greatly higher than that in contrast groups(P
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AIM:To explore the effect of the pretreatment of hypertonic saline(HTS) in hepatic ischemia reperfusion(I/R) injury.METHODS:The rats were divided into sham group(sham group),ischemia reperfusion group(IR group) and pretreatment of hypertonic saline group(HTS group).Partial hepatic ischemia reperfusion model was used.The rats were sacrificed at the time of 1 h,3 h,6 h,12 h and 24 h after reperfusion in each group,respectively.Blood samples were obtained to examine ALT.The expression of the CD11b/CD18(Mac-1) on the neutrophils was analyzed by flow cytometry.RT-PCR and Western blotting were used to examine the expression of intercellular adhesion molecule-1(ICAM-1) in livers and chromatometry was performed to detect the activity of myeloperoxidase(MPO) in livers.The morphology of hepatocytes and the structure of sinusoid were observed by histological examinations.RESULTS:① HTS pretreatment decreased the level of ALT at the time points of 3 h,6 h and 12 h after reperfusion(P
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PURPOSE: Fc receptors and Mac-1 play an important role in the protective response of granulocytes and monocytes against microbial infection. FcgammaRl, FcgammaRll, FcgammaRlll as well as CD11b/ CD18 have never been measured in a quantitative way during hemopoiesis. Thus we quantified the expression of Fc Rl, Fc Rll, Fc Rlll, and CD11b/CD18 during hematopoietic differentiation using HL-60 cells, which was induced to differentiate by DMSO, or PMA. METHODS: HL-60 cells (ATCC CCL-240) were induced to differentiate by adding 1.0% DMSO, or 16nM PMA. On the 4th and 7th day after stimulation as well as before stimulation, phenotypic analysis was performed by flow cytometry after staining the cells with PE-conjugated anti- human CD64, CD32, CD16, CD11b, CD18, and isotype controls. And the measured fluorescent intensity was transformed into Molecules of Equivalent Soluble Fluorochromes (MESF). RESULTS: Percent positive cells and MESF of CD11b on HL-60 cells increased upon induction by DMSO, but not by PMA. Percent positive cells of CD18 on HL-60 cells was 99% regardless of differentiation. But MESF of CD18 was increased on the 4th day and decreased on the 7th day by DMSO or PMA. Percent positive cells and MESF of FcgammaRl on HL-60 cells increased upon induction by DMSO or PMA. Percent positive cells of FcgammaRll on HL-60 cells was above 90% regardless of differentiation. MESF of FcgammaRll showed no significant change by DMSO or PMA. CONCLUSION: Quantitative expression of FcgammaRl, FcgammaRll, FcgammaRlll, and CD11b/CD18 of HL-60 cells changed during induction of differentiation by DMSO or PMA. MESF of FcgammaR and CD11b/ CD18 a better indicator than percent positive cells to compare the differentiation of HL-60cells.
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Humans , Dimethyl Sulfoxide , Flow Cytometry , Fluorescent Dyes , Granulocytes , HL-60 Cells , Monocytes , Receptors, FcABSTRACT
To investigate the role of ICAM 1 in the aggregation of polymorphoneucleocytes (PMN) in the lungs at the early stage of burns. Myeloperoxidase (MPO) content in lung tissues and bronchoalveolar lavage fluid (BALF) was assayed. Intercellular adhesion molecule 1 (ICAM 1) and its mRNA expression in lung tissues were determined by immunohistochemical method and in situ hybridization. CD11b/CD18 expression on the circulating PMN were measured by flow cytometry. The results showed that levels of MPO in lung tissues and BALF were markedly higher than those of control after burn injury. Expression of ICAM 1 and its mRNA in the lung tissues and CD11b/CD18 on circulating PMN surface were significantly increased at 2, 6, 12, 24hr after burns. It suggests that PMN aggregation in the lungs might be related to increased ICAM 1 expression on pulmonary microvascular endothelial cells and CD11b/CD18 expression on PMN at the early stage of burn injury.